Journal: Animals : an Open Access Journal from MDPI

Article Title: Intestinal Immune Cell Populations, Barrier Function, and Microbiomes in Broilers Fed a Diet Supplemented with Chlorella vulgaris

doi: 10.3390/ani13142380

Figure Lengend Snippet: Changes in the populations of immune cells in the jejunal lamina propria of 10-day-old broilers fed a control diet or a Chlorella vulgaris (CV)-containing diet. ( A ) The distribution of monocyte/macrophages (Mono/Macro) and B cells in MHCII cells; ( B ) populations of Mono/Macro and B cells in MHCII cells; ( C ) the distribution of CD8 and CD4 cells in CD3 cells; ( D ) populations of CD4 and CD8 T cells in CD3 cells; ( E ) the distribution of TCRγδ and CD8 cells in CD3 cells; ( F ) populations of TCRγδ T cells in CD3 cells. In figure ( A , C , E ), colors represent the density of cells in that location and indicate the density of cells in the order of red, yellow, green, and blue. Each data point represents six replicates and is presented as mean ± standard deviation. Significant differences between the treatments were analyzed using the 1-sample 2-tailed t -test. * p < 0.05.

Article Snippet: T cell subsets were stained with Pacific Blue-conjugated mouse anti-chicken CD3 (8200-26, Southern Biotech, Birmingham, AL, USA), FITC-conjugated mouse anti-chicken CD4 (8210-02, Southern Biotech, Birmingham, AL, USA), SPRD-conjugated mouse anti-chicken CD8α (8220-13, Southern Biotech, Birmingham, AL, USA), and PE-conjugated mouse anti-chicken TCRγδ antibodies (8230-09, Southern Biotech, Birmingham, AL, USA).

Techniques: Control, Standard Deviation

Journal: Viruses

Article Title: Comparative Analysis of Different Inbred Chicken Lines Highlights How a Hereditary Inflammatory State Affects Susceptibility to Avian Influenza Virus

doi: 10.3390/v15030591

Figure Lengend Snippet: Antibodies used for Flow Cytometry and Western Blotting.

Article Snippet: Mouse anti-chicken CD8α pacific blue , CT-8 , IgG1 , Southern Biotech , 1:1000 [ , ] .

Techniques: Flow Cytometry, Western Blot

Journal: Viruses

Article Title: Comparative Analysis of Different Inbred Chicken Lines Highlights How a Hereditary Inflammatory State Affects Susceptibility to Avian Influenza Virus

doi: 10.3390/v15030591

Figure Lengend Snippet: Differential γδ and αVβ1+ T cell subsets in the spleen of line 0 and line C.B21 birds. Flow cytometric analysis of cells isolated from the spleens of 4-week-old line 0 (black) and line C.B12 birds (red). To allow for the comparison of the numbers of cells between the chicken lines, 10,000 live single CD45 + cells were examined from each bird. ( a ) γδ T cell subsets expressing CD8αα+ or CD8αβ + and ( b ) αVβ1 + T cell subsets expressing CD8αα, CD8αβ, CD4 and CD4 + CD8α + . * p < 0.05, ** p < 0.01. Filled symbols represent experiment 1 and open symbols represent experiment 2; n = 10 line 0 birds and n = 9 line C.B12 birds. Black bar represents the mean. Error bars represent the standard deviation.

Article Snippet: Mouse anti-chicken CD8α pacific blue , CT-8 , IgG1 , Southern Biotech , 1:1000 [ , ] .

Techniques: Isolation, Expressing, Standard Deviation

Journal: Frontiers in Immunology

Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

doi: 10.3389/fimmu.2025.1517697

Figure Lengend Snippet: List of antibodies.

Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

Techniques: Control

Journal: Frontiers in Immunology

Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

doi: 10.3389/fimmu.2025.1517697

Figure Lengend Snippet: Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.

Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

Techniques: Flow Cytometry, Expressing

Journal: Frontiers in Immunology

Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

doi: 10.3389/fimmu.2025.1517697

Figure Lengend Snippet: LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).

Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

Techniques: Isolation, Selection, Cell Culture, Flow Cytometry, Staining, Labeling, Two Tailed Test

Journal: Frontiers in Immunology

Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

doi: 10.3389/fimmu.2025.1517697

Figure Lengend Snippet: List of antibodies.

Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

Techniques: Control